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KMID : 0377619650080060647
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Korean Jungang Medical Journal
1965 Volume.8 No. 6 p.647 ~ p.656
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Effect of Bloodletting on Water and Electroyte Distribution in Various Rabbit Tissue.
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Abstract
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Ninety rabbits were divided into 3 groups in order to study the effects of blood letting on water and electrolytes distribution in various tissues.
group 1. control, 30 rabbits
group 2. 20cc of blood letted, 30 rabbits
group 3. 40cc of blood le tted, 30 rabbits
The blood was depleted slowly by cardiac puncture. After 15 hours bloodletting the animals were killed and liver, kidney, intestine, spleen, skeletal muscle and heart were taken out to determine the contents of water, sodium, potassium and chloride.
Water content was determined by drying method in 100°C oven until the weight remained constant. Sodium and potassuim were analyzed by means of Baird flame photometer.
Serum chloride was analyzed after Schales and Schales, while tissue chloride after van Slyke.
The results of this investigation were as follows.
1) Sodium was decreased remarkably in serum, while the changes in potassium and chloride were not significant.
2) Sodium and potassium were decreased, but chloride and chloride space were increased in liver.
3) Sodium and sodium space were increased and potassuim was inversely decreased in Kidney.
4) In intestine, water, sodium, potassium, chloride, extracellular spaces and total Na^(+) + k^(+)were inclusively decreased.
5) Sodium, potassium and chloride in spleen were decreased, but water content was not changed.
6) In skeletal muscle tissue, sodium and sodium space were decreased significantly, while others were not changed remarkably.
7) Decrease of potassium was siginficant in cardiac muscle.
In general the changes of electrolytes contents were more remarkable in the animals 40cc of blood letted than in the animals 20cc of blood letted, and tissue water was not changed except in intestine.
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